Composite
Part:BBa_K4121082
Designed by: Ziyu Li Group: iGEM22_NEFU_China (2022-10-05)
Expression cassette of ERG10 with constitutive promoter
The part is called "pCCW12-ERG10-tCPS1". We use pCCW12 as the promoter, tCPS1 as the terminator and the CDS of this part is ERG10. The three basic parts above make up a new transcription unit, It has the function to catalyze the conversion of Acetyl-CoA to Acetoacetyl-CoA in mevalonate pathway.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 240
Illegal EcoRI site found at 1465
Illegal PstI site found at 1280 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 240
Illegal EcoRI site found at 1465
Illegal PstI site found at 1280 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 240
Illegal EcoRI site found at 1465
Illegal BglII site found at 1204 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 240
Illegal EcoRI site found at 1465
Illegal PstI site found at 1280 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 240
Illegal EcoRI site found at 1465
Illegal PstI site found at 1280 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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